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pf2-dta-rosa26  (Addgene inc)


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    Structured Review

    Addgene inc pf2-dta-rosa26
    Pf2 Dta Rosa26, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pf2-dta-rosa26/rosa26+locus/pmc03577784-48-68-78
    Average 90 stars, based on 1 article reviews
    pf2-dta-rosa26 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. coli
    Article Snippet: Correct clones were identified by restriction enzyme digestion of miniprep DNA and confirmed by sequencing with Fab-BstE2-F and Fab-BssH2-R. pF-DTA was generated by recombineering with fragments from pDTA, which has the EF1a-DTA negative selection marker cloned into pBS backbone, cut by Apa LI deleting ampicillin resistance marker and pF by Afl III/ Dra III containing fabI . pF2-DTA was generated by cloning mfabI into pF-DTA by Fsp I/ Apa LI. pF2-DTA-Rosa26 was generated by inserting fragment from pRosa26-1 (Addgene plasmid 21714) cut by Eco 53kI/ Sal I into the Nru I/ Sal I sites in pF2-DTA.

    Sequencing:

    Article Title: A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. coli
    Article Snippet: Correct clones were identified by restriction enzyme digestion of miniprep DNA and confirmed by sequencing with Fab-BstE2-F and Fab-BssH2-R. pF-DTA was generated by recombineering with fragments from pDTA, which has the EF1a-DTA negative selection marker cloned into pBS backbone, cut by Apa LI deleting ampicillin resistance marker and pF by Afl III/ Dra III containing fabI . pF2-DTA was generated by cloning mfabI into pF-DTA by Fsp I/ Apa LI. pF2-DTA-Rosa26 was generated by inserting fragment from pRosa26-1 (Addgene plasmid 21714) cut by Eco 53kI/ Sal I into the Nru I/ Sal I sites in pF2-DTA.

    Generated:

    Article Title: A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. coli
    Article Snippet: Correct clones were identified by restriction enzyme digestion of miniprep DNA and confirmed by sequencing with Fab-BstE2-F and Fab-BssH2-R. pF-DTA was generated by recombineering with fragments from pDTA, which has the EF1a-DTA negative selection marker cloned into pBS backbone, cut by Apa LI deleting ampicillin resistance marker and pF by Afl III/ Dra III containing fabI . pF2-DTA was generated by cloning mfabI into pF-DTA by Fsp I/ Apa LI. pF2-DTA-Rosa26 was generated by inserting fragment from pRosa26-1 (Addgene plasmid 21714) cut by Eco 53kI/ Sal I into the Nru I/ Sal I sites in pF2-DTA.

    Selection:

    Article Title: A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. coli
    Article Snippet: Correct clones were identified by restriction enzyme digestion of miniprep DNA and confirmed by sequencing with Fab-BstE2-F and Fab-BssH2-R. pF-DTA was generated by recombineering with fragments from pDTA, which has the EF1a-DTA negative selection marker cloned into pBS backbone, cut by Apa LI deleting ampicillin resistance marker and pF by Afl III/ Dra III containing fabI . pF2-DTA was generated by cloning mfabI into pF-DTA by Fsp I/ Apa LI. pF2-DTA-Rosa26 was generated by inserting fragment from pRosa26-1 (Addgene plasmid 21714) cut by Eco 53kI/ Sal I into the Nru I/ Sal I sites in pF2-DTA.

    Marker:

    Article Title: A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. coli
    Article Snippet: Correct clones were identified by restriction enzyme digestion of miniprep DNA and confirmed by sequencing with Fab-BstE2-F and Fab-BssH2-R. pF-DTA was generated by recombineering with fragments from pDTA, which has the EF1a-DTA negative selection marker cloned into pBS backbone, cut by Apa LI deleting ampicillin resistance marker and pF by Afl III/ Dra III containing fabI . pF2-DTA was generated by cloning mfabI into pF-DTA by Fsp I/ Apa LI. pF2-DTA-Rosa26 was generated by inserting fragment from pRosa26-1 (Addgene plasmid 21714) cut by Eco 53kI/ Sal I into the Nru I/ Sal I sites in pF2-DTA.

    Cloning:

    Article Title: A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. coli
    Article Snippet: Correct clones were identified by restriction enzyme digestion of miniprep DNA and confirmed by sequencing with Fab-BstE2-F and Fab-BssH2-R. pF-DTA was generated by recombineering with fragments from pDTA, which has the EF1a-DTA negative selection marker cloned into pBS backbone, cut by Apa LI deleting ampicillin resistance marker and pF by Afl III/ Dra III containing fabI . pF2-DTA was generated by cloning mfabI into pF-DTA by Fsp I/ Apa LI. pF2-DTA-Rosa26 was generated by inserting fragment from pRosa26-1 (Addgene plasmid 21714) cut by Eco 53kI/ Sal I into the Nru I/ Sal I sites in pF2-DTA.

    Plasmid Preparation:

    Article Title: A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. coli
    Article Snippet: Correct clones were identified by restriction enzyme digestion of miniprep DNA and confirmed by sequencing with Fab-BstE2-F and Fab-BssH2-R. pF-DTA was generated by recombineering with fragments from pDTA, which has the EF1a-DTA negative selection marker cloned into pBS backbone, cut by Apa LI deleting ampicillin resistance marker and pF by Afl III/ Dra III containing fabI . pF2-DTA was generated by cloning mfabI into pF-DTA by Fsp I/ Apa LI. pF2-DTA-Rosa26 was generated by inserting fragment from pRosa26-1 (Addgene plasmid 21714) cut by Eco 53kI/ Sal I into the Nru I/ Sal I sites in pF2-DTA.



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